Interdisciplinary Bio Central
 
Full Report (Resources)

Genetic Quality Control of the Rat Strains at the National Bio Resource Project - Rat
Takashi Kuramoto1,*, Satoshi Nakanishi1, Ken-ichi Yamasaki1, Kenta Kumafuji1, Yuichi Sakakibara1, Yuki Neoda1, Akiko Takizawa1, Takehito Kaneko1, Mito Otsuki1, Ryoko Hashimoto1, Birger Voigt1, Tomoji Mashimo1 and Tadao Serikawa1,*
1Institute of Laboratory Animals, Graduate School of Medicine, Kyoto University, Yoshidakonoe-cho, Sakyo-ku, Kyoto 606-8501, Japan
*Corresponding author
  Received : November 12, 2010
  Revised : November 19, 2010
  Accepted : November 19, 2010
  Published : November 23, 2010
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Main text PDF(4114KB)
   (Print version)
Synopsis

The National Bio Resource Project - Rat (NBRP-Rat) comprises the largest bank of laboratory rat (Rattus norvegicus) strains in the world. Its main focus is to develop infrastructure that will facilitate the systematic collection, preservation, and provision of rat strains. To breed effectively more than 180 rat strains in living stock, we establish the genetic control system in which a systematic set of genetic diagnoses and genetic monitoring are included. Genetic monitoring is performed by using 20 polymorphic markers. Monitoring is carried out when a living animal stock is re-established by using cryopreserved embryos or sperm or when a rat strain is first introduced to the NBRP-Rat by a depositor. Additional monitoring is then carried out on each strain every two years. Genetic diagnosis is performed largely by employing the Amp-FTA method. Protocols which detail how to perform a genetic diagnosis of 11 transgenes and 24 mutations have been made. Among the mutations, nine can be detected by simple gel electrophoresis of the PCR products, 11 by restriction enzyme treatment of the PCR products, and four by direct PCR product sequencing. Using this genetic control system, the NBRP-Rat can guarantee the genetic quality of its rat strains.

Keyword: rat, National Bio Resource Project, strain, genotyping, genetic monitoring, transgenic rat, mutation, disease model, Hermansky-Pudlak syndrome 5, Amp-FTA method
IBC   ISSN : 2005-8543   Contact IBC