Interdisciplinary Bio Central
 
Review (Cheminformatics/Chemical biology)

Target Identification : A Challenging Step in Forward Chemical Genetics
Raj Kumar Das1, Animesh Samanta1, Krishnakanta Ghosh2, Duanting Zhai2, Wang Xu2, Dongdong Su2, Cheryl Leong3 and Young-Tae Chang1,2,4,*
1Department of Chemistry, National University of Singapore, 117543 Singapore
2Department of Chemistry & MedChem Program of Life Sciences Institute, National University of Singapore, 117543 Singapore
3Graduate School for Integrative Sciences and Engineering, National University of Singapore, 119077 Singapore
4Singapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), 11 Biopolis Way, 138667 Singapore
*Corresponding author
  Received : January 21, 2011
  Accepted : January 21, 2011
  Published : January 26, 2011
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Main text PDF(6639KB)
   (Print version)
Synopsis

Investigation of the genetic functions in complex biological systems is a challenging step in recent year. Hence, several valuable and interesting research projects have been developed with novel ideas to find out the unknown functions of genes or proteins. To validate the applicability of their novel ideas, various approaches are built up. To date, the most promising and commonly used approach for discovering the target proteins from biological system using small molecule is well known a forward chemical genetics which is considered to be more convenient than the classical genetics. Although, the forward chemical genetics consists of the three basic components, the target identification is the most challenging step to chemical biology researchers. Hence, the diverse target identification methods have been developed and adopted to disclose the small molecule bound protein. Herein, in this review, we briefly described the first two parts chemical toolbox and screening, and then the target identifications in forward chemical genetics are thoroughly described along with the illustrative real example case study. In the tabular form, the different biological active small molecules which are the successful examples of target identifications are accounted in this research review.

Keyword: chemical toolbox, small molecules, screening, cell imaging, phenotype change, genetic information, proteomes,
IBC   ISSN : 2005-8543   Contact IBC