Interdisciplinary Bio Central
 
Experimental method/Instrumentation (Bioimaging)

Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Orthogonal Fluorescent Compound
Jae-Jung Lee1+, Jun-Young Kim2+, Duanting Zhai2, Seong-Wook Yun1 and Young-Tae Chang1,2,*
1Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research, Biopolis, Singapore
2Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore, Singapore
*Corresponding author
+These authors contributed equally to this work
  Received : June 01, 2012
  Accepted : June 12, 2012
  Published : June 15, 2012
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Synopsis

Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose 쏣stern staining, as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming 쐇mmuno-staining in many versatile applications.

Keyword: Western blot, Estern staining, fluorescence dye BDED, RC-tag, peptide chemical tags
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